Optimasi Carboxymethyl Cellulose Dan Hyaluronic Acid Sebagai Hydrogel Untuk Aplikasi Wound Dressing Pada Luka Bakar
Abstrak
Luka bakar merupakan cedera kulit yang disebabkan oleh paparan panas atau bahan kimia menjadi tantangan serius dalam kesehatan masyarakat secara global. Berdasarkan data WHO, prevalensi luka bakar mencapai lebih dari 11 juta kasus yang memerlukan penanganan medis setiap tahun. Salah satu pendekatan untuk mempercepat proses penyembuhan luka adalah dengan menjaga kelembapan menggunakan wound dressing. Hydrogel sebagai wound dressing menjadi salah satu pilihan yang menarik karena kemampuannya mempertahankan kadar air yang tinggi yaitu 70–90%, sehingga menciptakan lingkungan lembap yang ideal untuk penyembuhan luka. Penelitian ini bertujuan untuk mengembangkan amorphous hydrogel berbasis carboxymethyl cellulose (CMC) dan hyaluronic acid (HA), yang diformulasikan untuk menyesuaikan luka dengan bentuk tidak teratur. CMC dipilih karena sifat hidrofiliknya yang mampu meningkatkan stabilitas mekanik hydrogel, sedangkan HA berperan dalam mendukung regenerasi jaringan, menjaga hidrasi, dan memberikan efek antimikroba. Pembuatan hydrogel dilakukan dengan mencampurkan HA 2,5% dan CMC 5% dengan variasi rasio 1:1, 1:3, 1:5 (v/v) dan HA 2,5% sebagai kontrol. Karakterisasi dilakukan melalui uji viskositas, afinitas cairan, stabilitas, dan daya sebar. Hasil penelitian menunjukkan bahwa formulasi hydrogel dengan rasio HA:CMC 1:1 memberikan performa terbaik, dengan viskositas sebesar 81.120 cps, daya serap cairan mencapai 34,56%, serta stabilitas dan daya sebar yang mendukung aplikasi topikal. Dengan demikian, formulasi ini berpotensi sebagai wound dressing efektif untuk mendukung penyembuhan luka bakar secara optimal. Kata Kunci : amorphous hydrogel, carboxymethyl cellulose (CMC), hyaluronic acid, luka bakar, wound dressing
Referensi
[1] K. A. Cook, E. Martinez-Lozano, R. Sheridan, E. K. Rodriguez, A. Nazarian, and M. W. Grinstaff, “Hydrogels for the management of second-degree burns: currently available options and future promise,” Burn. Trauma, vol. 10, 2022, doi: 10.1093/burnst/tkac047.
[2] A. A. W. Perkasa-hendropriyono and I. D. Saputro, “Contribution of SIRS and sepsis on the outcomes of patients with burn injuries in Dr . Soetomo Teaching Hospital,” vol. 14, no. 1, pp. 197–202, 2025, doi: 10.15562/bmj.v14i1.5406.
[3] A. Wardhana and G. A. Winarno, “Epidemiology And Mortality Of Burn Injury In Ciptomangunkusumo Hospital, Jakarta: A 5 Year Retrospective Study,” J. Plast. Rekonstruksi, vol. 6, no. 1, pp. 234–242, 2020, doi: 10.14228/jpr.v6i1.270.
[4] H. Wang et al., “Antibacterial polysaccharide- based hydrogel dressing containing plant essential oil for burn wound healing,” Burn. Trauma, vol. 9, pp. 1–14, 2021, doi: 10.1093/burnst/tkab041.
[5] W. Shu, Y. Wang, X. Zhang, C. Li, H. Le, and F. Chang, “Functional Hydrogel Dressings for Treatment of Burn Wounds,” Front. Bioeng. Biotechnol., vol. 9, no. December, pp. 1–18,
2021, doi: 10.3389/fbioe.2021.788461.
[6] J. Xiao, Y. Liang, T. Sun, M. Liu, and X. He, “A functional dual responsive CMC/OHA/SA/TOB hydrogel as wound dressing to enhance wound healing,” Sci. Rep., vol. 14, no. 1, p. 26854, 2024, doi: 10.1038/s41598-024-78044-8.
[7] A. Surowiecka, J. Strużyna, A. Winiarska, and T. Korzeniowski, “Hydrogels in Burn Wound Management—A Review,” Gels, vol. 8, no. 2, 2022, doi: 10.3390/gels8020122.
[8] Y. Hong, Z. Lin, Y. Yang, T. Jiang, J. Shang, and
Z. Luo, “Biocompatible Conductive Hydrogels: Applications in the Field of Biomedicine,” Int. J. Mol. Sci., vol. 23, no. 9, 2022, doi: 10.3390/ijms23094578.
[9] C. Xie et al., “Synthesis and Properties of Injectable Hydrogel for Tissue Filling,” Pharmaceutics, vol. 16, no. 3, 2024, doi: 10.3390/pharmaceutics16030430.
[10] H. Li et al., “Injectable, self-healing, antibacterial, and hemostatic N,O-carboxymethyl chitosan/oxidized chondroitin sulfate composite hydrogel for wound dressing,” Mater. Sci. Eng. C, vol. 118, no. August 2020, p. 111324, 2021, doi: 10.1016/j.msec.2020.111324.
[11] Y. C. Huang, K. Y. Huang, B. Y. Yang, C. H. Ko, and H. M. Huang, “Fabrication of Novel Hydrogel with Berberine-Enriched Carboxymethylcellulose and Hyaluronic Acid as an Anti-Inflammatory Barrier Membrane,” Biomed Res. Int., vol. 2016, 2016, doi: 10.1155/2016/3640182.
[12] W. Zhang, Y. Liu, Y. Xuan, and S. Zhang, “Synthesis and Applications of Carboxymethyl Cellulose Hydrogels,” Gels, vol. 8, no. 9, 2022, doi: 10.3390/gels8090529.
[13] A. Song, “Exploring Hyaluronic Acid as a Potential Standard Dressing for Burn Wound,” Int.
J. Med. Sci. Clin. Res. Stud., vol. 02, no. 08, 2022, doi: 10.47191/ijmscrs/v2-i8-13.
[14] F. Fayyazbakhsh, M. J. Khayat, and M. C. Leu, “3D-Printed Gelatin-Alginate Hydrogel Dressings for Burn Wound Healing: A Comprehensive Study,” Int. J. Bioprinting, vol. 8, no. 4, pp. 274– 291, 2022, doi: 10.18063/ijb.v8i4.618.
[15] A. K. Zusandy, Arwi Amiruddin, Andi Sastri Z, Lisa Yuniati, and Andi Miranti, “Gambaran dan Karakteristik Pasien Luka Bakar,” Fakumi Med. J.
J. Mhs. Kedokt., vol. 4, no. 5, pp. 412–422, 2024, doi: 10.33096/fmj.v4i5.455.
[16] G.-E. Ja et al., “Burns: Definition, Classification, Pathophysiology and Initial Approach,” no. June, 2020, doi: 10.4172/2327-5146.1000298.
[17] H. M. Nguyen, T. T. Ngoc Le, A. T. Nguyen, H. N. Thien Le, and T. T. Pham, “Biomedical materials for wound dressing: recent advances and applications,” RSC Adv., vol. 13, no. 8, pp. 5509–
5528, 2023, doi: 10.1039/d2ra07673j.
[18] R. C. Opt Veld, X. F. Walboomers, J. A. Jansen, and F. A. D. T. G. Wagener, “Design Considerations for Hydrogel Wound Dressings:
Strategic and Molecular Advances,” Tissue Eng.
- Part B Rev., vol. 26, no. 3, pp. 230–248, 2020, doi: 10.1089/ten.teb.2019.0281.
[19] V. Brumberg, T. Astrelina, T. Malivanova, and
A. Samoilov, “Modern wound dressings: Hydrogel dressings,” Biomedicines, vol. 9, no. 9, 2021, doi: 10.3390/biomedicines9091235.
[20] E. Rezvani Ghomi, S. Khalili, S. Nouri Khorasani, R. Esmaeely Neisiany, and S. Ramakrishna, “Wound dressings: Current advances and future directions,” J. Appl. Polym. Sci., vol. 136, no. 27, pp. 1–12, 2019, doi: 10.1002/app.47738.
[22] H. Haryanto, “Pengaruh Kitosan Terhadap Karakterisasi Hidrogel Film PVA Untuk Aplikasi Pembalut Luka,” Techno (Jurnal Fak. Tek. Univ. Muhammadiyah Purwokerto), vol. 22, no. 2, p. 123, 2021, doi:
10.30595/techno.v22i2.11593.
[21] E. M. Ahmed, “Hydrogel: Preparation, characterization, and applications: A review,” J. Adv. Res., vol. 6, no. 2, pp. 105–121, 2015, doi: 10.1016/j.jare.2013.07.006.
[22] J. M. Alonso, J. A. Del Olmo, R. P. Gonzalez, and V. Saez‐martinez, “Injectable hydrogels: From laboratory to industrialization,” Polymers (Basel)., vol. 13, no. 4, pp. 1–24, 2021, doi: 10.3390/polym13040650.
[23] M. Vivi Purwandari, Mahyuni Harahap1, Liver Imam Putra Zai1 and A. Mubarakh1, “Jurnal Kimia Saintek dan Pendidikan,” J. Kim. Saintek dan Pendidik., vol. 7, no. 1, pp. 72–78, 2023.
[24] M. S. Rahman et al., “Recent developments of carboxymethyl cellulose,” Polymers (Basel)., vol. 13, no. 8, 2021, doi: 10.3390/polym13081345.
[25] V. Kanikireddy, K. Varaprasad, T. Jayaramudu,
C. Karthikeyan, and R. Sadiku, “Carboxymethyl cellulose-based materials for infection control and wound healing: A review,” Int. J. Biol. Macromol., vol. 164, pp. 963–975, 2020, doi: 10.1016/j.ijbiomac.2020.07.160.
[26] T. I. Sari, P. Susmanto, M. H. Dahlan, N. I. Kamega, and A. Pratiwi, “Jurnal Integrasi Proses Website: http://jurnal.untirta.ac.id/index.php/jip Pembuatan Hidrogel Berbasis Polivinil Alkohol (PVA)/Karboksimetil Selulosa (CMC)/Minyak Asiri Serai menggunakan Metode Chemical Crosslinked,” J. Integr. Proses, vol. 13, no. 1, pp. 43–51, 2024, [Online]. Available: http://jurnal.untirta.ac.id/index.php/jip
[27] M. G. Neuman, R. M. Nanau, L. Oruña-Sanchez, and G. Coto, “Hyaluronic acid and wound healing,” J. Pharm. Pharm. Sci., vol. 18, no. 1,
pp. 53–60, 2015, doi: 10.18433/j3k89d.
[28] O. Dereure, M. Czubek, and P. Combemale, “Efficacy and safety of hyaluronic acid in treatment of leg ulcers: A double-blind RCT,” J. Wound Care, vol. 21, no. 3, pp. 131–139, 2012, doi: 10.12968/jowc.2012.21.3.131.
[29] A. Yasin, Y. Ren, J. Li, Y. Sheng, C. Cao, and K. Zhang, “Advances in Hyaluronic Acid for Biomedical Applications,” Front. Bioeng. Biotechnol., vol. 10, no. July, pp. 1–12, 2022, doi: 10.3389/fbioe.2022.910290.
[30] M. M. Dalmedico, M. J. Meier, J. V. C. Felix, F. S. Pott, F. de F. C. Petz, and M. C. Santos, “Hyaluronic acid covers in burn treatment: A systematic review,” Rev. da Esc. Enferm., vol. 50, no. 3, pp. 519–524, 2016, doi: 10.1590/S0080-
623420160000400020.
[31] A. D. Djajanti, I. N. P. Sumule, Firmansyah, and Rusli, “Formulation And Physical Stability Test Of Celery Leaf Extract Gel (Apium graveolens L .) With Variations Concentration Of Hydroxy Propyl Methyl Cellulose And Carbopol,” 3rd Int. Conf. Urban Heal. Covid-19 Pandemic amd Urban Heal. Issues, vol. 3, no. 1, pp. 54–63, 2021.
[32] W. Wulandari, D. E. Ermawati, and A. Yugatama, “Optimization SNEDDS (Self-Nano Emulsifying Drug Delivery System) of ZnO that dispersed into Hydrogel Matrix as UV-Protective,” IOP Conf. Ser. Mater. Sci. Eng., vol. 578, no. 1, 2019, doi: 10.1088/1757-899X/578/1/012058.
[33] A. Aliyah, W. W. Oktaviana, K. S. Dwipayanti, A.
P. Erdiana, R. N. Utami, and A. D. Permana, “Enhanced skin localization of doxycycline using microparticles and hydrogel: Effect of oleic acid as penetration enhancer,” Pharmaciana, vol. 11, no. 2, p. 239, 2021, doi:
10.12928/pharmaciana.v11i2.21044.
[34] S. Url, C. Press, T. J. Archive, and T. J. Archive, Encyclopedia of Geoarcheology, vol. 23, no. 1998. 2007.
[35] J. Grip et al., “Development of a novel beta-glucan supplemented hydrogel spray formulation and wound healing efficacy in a db/db diabetic mouse model,” Eur. J. Pharm. Biopharm., vol. 169, no. October, pp. 280–291, 2021, doi: 10.1016/j.ejpb.2021.10.013.
[36] J. K. Indonesia and N. A. Sayuti, “Artikel Riset Formulasi dan Uji Stabilitas Fisik Sediaan Gel Ekstrak Daun Ketepeng Cina ( Cassia alata L .) Formulation and Physical Stability of Cassia alata L . Leaf Extract Gel penyakit yang menyerang pada permu- Malassezia furfur . Penyakit yang diseb,” vol. 5, no. 2, pp. 74–82, 2015.
[37] A. Indalifiany, M. Hajrul Malaka, A. Fristiohady,
R. Andriani, and M. Harul Malaka, “Jurnal Farmasi Sains dan Praktis Formulasi dan Uji Stabilitas Fisik Nanoemulgel Ekstrak Etanol Spons Petrosia Sp. Formulation and Physical Stability Test of Nanoemulgel Containing Petrosia Sp. Ethanolic Extract,” Jfsp, vol. 7, no. 3, pp. 2579–
4558, 2021, [Online]. Available: http://journal.ummgl.ac.id/index.php/pharmacy
[38] T. Imanto, R. Prasetiawan, and E. R. Wikantyasning, “Formulasi dan Karakterisasi Sediaan Nanoemulgel Serbuk Lidah Buaya (Aloe Vera L.),” Pharmacon J. Farm. Indones., vol. 16,
no. 1, pp. 28–37, 2019, doi: 10.23917/pharmacon.v16i1.8114.
[39] M. Diki Wahyudi et al., “Formulasi dan Uji Stabilitas Fisik Gel Ekstrak Batang Pisang Mauli (Musa acuminata),” Dentin J. Kedokt. Gigi, vol. 2022, no. 3, pp. 161–165, 2022.
